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Improvement of reproductive performance of clown anemonefish, Amphirion ocellaris (Cuvier, 1830), with vitamin C

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dc.contributor.author Ho, Son Lam
dc.contributor.author Nguyen, Thi Nguyet Hue
dc.contributor.author Dao, Thi Hong Ngoc
dc.contributor.author Dang, Tran Tu Tram
dc.contributor.author Do, Hai Dang
dc.contributor.author Nguyen, Truong Tan Tai
dc.contributor.author Hoang, Xuan Ben
dc.contributor.author Dao, Viet Ha
dc.contributor.author Pham, T Khanh
dc.contributor.author Nguyen, Thi My Ngan
dc.contributor.author Phan, Minh Thu
dc.date.accessioned 2025-08-29T08:05:37Z
dc.date.available 2025-08-29T08:05:37Z
dc.date.issued 2025
dc.identifier.issn 1844-8143
dc.identifier.uri http://tvhdh.vnio.org.vn:8080/xmlui/handle/123456789/21564
dc.description.abstract An enhancement in broodstock nutrition can significantly influence the reproductive success of fish species. This study investigated the impact of vitamin C supplementation in broodfish diets on the reproductive performance, egg quality, and larvae parameters of clown anemonefish (Amphiprion ocellaris). Five diet treatments with varying levels of vitamin C in form of L-Ascorbyl-2-Polyphosphate (0.0, 200, 400, 600, and 800 mg kg-1 diet) were implemented over a 12-month period. Results indicated positive effects of vitamin C supplementation on fecundity, egg quality, hatching rate, larvae survival, and malformation rate (p<0.05). The treatment with 600 mg kg-1 diet exhibited the highest hatching rate (94.61%), lowest egg loss rate (12.86%), and lowest malformation rate (0.519%). Egg diameter and larval size did not significantly differ among treatments (p>0.05). Although vitamin C supplementation reduced re-maturation time and increased spawning frequency, the differences were not statistically significant (p>0.05). Overall, a dietary supplementation of 600 mg kg-1 vitamin C is recommended to improve reproductive performance in clownfish broodstock. vi,en
dc.language.iso en vi,en
dc.relation.ispartofseries AACL Bioflux, 2025, Volume 18, Issue 1, pp. 185-199;
dc.subject Clown anemonefish vi,en
dc.subject Amphirion ocellaris vi,en
dc.subject Malformed rate vi,en
dc.subject Reproductive quality vi,en
dc.subject Survival rate vi,en
dc.subject Vitamin C vi,en
dc.title Improvement of reproductive performance of clown anemonefish, Amphirion ocellaris (Cuvier, 1830), with vitamin C vi,en
dc.type Working Paper vi,en


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